Solid Samples (Soil and soil blends, biologicals)

***Due to the COVID-19 crisis, we are unable to accept samples at this time. This page will be updated when we are able to accept samples again. Email zearley.1@osu.edu if you would like to be notified of the lab's reopening via email.***

pH and electrical conductivity 

Analysis Method (Click the method to view the SOP) Self-serve Instrument Available Contact
pH 1:1  or 1:2 deionized water:media Yes Shane Whitacre
Salinity Electrical conductivity (1:1 deionized water:media) Yes Shane Whitacre

Total Elemental Analysis                  

Analysis Method (Click the method to view the SOP) Self-serve Instrument Available Contact
As, Cd, and Pb X-ray fluorescence screen (soil and inorganic only) N/A Yes Shane Whitacre
Anions by IC (F, Cl, NO2, NO3, PO4, SO4) Anion Extraction + USEPA 300.0 Extraction Only Shane Whitacre
Acid digestion+ ICP (soil or biological tissue) USEPA 3051a Extraction only Shane Whitacre
Multi-element X-ray fluorescence (soil and inorganic only) USEPA 6200 Yes Shane Whitacre

Nutrient Analysis

Analysis Method (Click the method to view the SOP) Self-serve Instrument Available Contact
Extractable K, Ca, Mg, Na; Base saturation; CEC Soil pH > 7.2:  ammonium acetate + ICP Extraction only Shane Whitacre
Soil pH < 7.2: Mehlich 3 + ICP Extraction only
Extractable P Soil pH >7.2: Olsen (sodium bicarbonate) + ICP Extraction only Shane Whitacre
Soil pH < 7.2: Mehlich 3 + ICP Extraction only
Soil pH < 7.2: Bray P + ICP Extraction only
Extractable Fe, Zn, Cu, Mn Soil pH > 7.2: DTPA  + ICP Extraction only Shane Whitacre
Soil pH < 7.2: Mehlich 3 + ICP Extraction only
Al, Fe Acid ammonium oxalate + ICP Extraction only Shane Whitacre
Plant available N 2M KCl (NH4+NO3) + Lachat colorimetric analysis Extraction only Shane Whitacre
Potentially mineralizable N  7 day incubation+ KCl extraction + Lachat colorimetric analysis Extraction only Shane Whitacre

Carbon and Nitrogen

Analysis Method (Click the method to view the SOP) Self-serve Instrument Available Contact
Total carbon and nitrogen Dry combustion Preparation only Richard Dick
Active carbon Potassium permanganate oxidation + colorimetric analysis Yes Steve Culman
Organic carbon Dichromate reduction + colorimetric analysis Yes Shane Whitacre
Total carbon    Loss on Ignition Preparation only Matt Davies
Inorganic C Calcimeter Yes Scott Demyan
Soil respiration 24-hour CO2 burst   Steve Culman
Soil protein Autoclaved citrate extractable protein   Steve Culman

Heavy Metal availability

Analysis Method (Click the method to view the SOP) Self-serve instrument available Contact
Human gastrointestinal bioaccessible Pb: Untreated soil USEPA 1340 + ICP   Shane Whitacre
Human gastrointestinal bioaccessible Pb: Treated soil Modified USEPA 1340 at pH 2.5 + ICP   Shane Whitacre
OSU In Vitro Gastrointestinal method (OSU IVG) + ICP   Shane Whitacre
Mammal, avian gastrointestinal bioaccessible Pb Modified USEPA 1340 at pH 2.5 + ICP   Shane Whitacre
OSU In Vitro Gastrointestinal method (OSU IVG) + ICP   Shane Whitacre
Plant bioaccessible Pb Neutral salt extraction (2M KCl) + ICP Extraction only Shane Whitacre
Human gastrointestinal bioaccessible As USEPA 1340 + ICP   Shane Whitacre
OSU In Vitro Gastrointestinal method (OSU IVG) + ICP   Shane Whitacre
Synthetic precipitation leaching procedure USEPA 1312 + ICP Extraction only Shane Whitacre
Human gastrointestinal bioaccessible As, high Fe soils California Arsenic Bioaccessibility method (CAB) + ICP   Shane Whitacre
Toxicity characteristic leaching procedure USEPA 1311 + ICP Extraction only Shane Whitacre

soil health measures and packages

Analysis Method (Click the method to view the SOP) Self-serve Instrument Available Contact
Soil Health Institute Extractable K, Ca, Mg, Na; Base saturation; CEC   Shane Whitacre
Extractable P
Extractable Fe, Zn, Cu, Mn
Potentially mineralizable N 
Organic carbon
Soil texture
Aggregate stability
pH
EC
Sodium absorption ratio
Active Organic Matter Active C   Steve Culman
Soil respiration
Soil protein
Active OM + Nutrients Active C   Steve Culman
Soil respiration
Soil protein
pH
Buffer pH
CEC
Organic matter (LOI)
M3 nutrients

physical measures and other analyses 

Analysis Method (Click the method to view the SOP) Self-serve Instrument Available Contact
Soil texture Pipette method (%sand, silt, clay) Yes Shane Whitacre
Wet aggregate stability Wet sieving apparatus Yes Shane Whitacre
Rainfall aggregate stability Rainfall simulator   Steve Culman
Soil stability index Dry + wet aggregate stability Yes Shane Whitacre
Oxalate extraction (Al, Fe) Acid ammonium oxalate + ICP Extraction only Shane Whitacre
P sorption 5 point P sorption isotherm + ICP   Shane Whitacre